علوم زیستی دریا
Amin Oujifard; Ahmad Shadi; Elaheh Fateh; Seyed Javad Hosseini
Abstract
Sillaginidae family fish from Perciforms, are appropriate candidates for shallow water and coastal aquaculture. At least three species of this family represents in the Persian Gulf. Genetic analysis of Sand whiting Sillago sihama , the most common species of family performed using COI gene. During the ...
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Sillaginidae family fish from Perciforms, are appropriate candidates for shallow water and coastal aquaculture. At least three species of this family represents in the Persian Gulf. Genetic analysis of Sand whiting Sillago sihama , the most common species of family performed using COI gene. During the present study 10 samples were collected from Bushehr and Hormozgan provinces coastal waters. DNA extracted by modified CTAB method. Polymerase chain reactions were performed using universal primers - FISHF1 and FISHR1-. Sequencing results showed a 627 bp amplified fragment. Performing BLAST supported high identity to Sillago sihama species, hence morphometric identification confirms molecular barcoding. Genetic distance of 0.02 was calculated between samples of two areas based on Kimura 2- parameter using Mega software. Constructed phylogenetic tree using neighbor joining method whereas the Acanthopagrus latus sequences was used as an outgroup revealed no differentiation between two stations samples. In conclusion based on the results of the present study, the gene flow was high among studied samples and no significant differentiation was observed between Bushehr and Hormozgan samples. In conclusion no discrete populations differentiated based on the results of the present study.
علوم زیستی دریا
Ahmad Shadi; Omid Pirnia
Abstract
Cultured ornamental fish are often faint coloured due to extensive culture conditions . Experiments using algae as food additive to the diets of fish resulted in colour enhancement due to their natural colourant compounds. Effect of Spirulina microalgae Arthrospira maxim diet on colour enhancement of ...
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Cultured ornamental fish are often faint coloured due to extensive culture conditions . Experiments using algae as food additive to the diets of fish resulted in colour enhancement due to their natural colourant compounds. Effect of Spirulina microalgae Arthrospira maxim diet on colour enhancement of Zebra Malawi cichlid Pseudotropheus zebra was investigated. Total 180 fish were tested in 6 treatments with tree replicate for each (control, 5%, 10%, 15%, 20% and 25% of dry Spirulina in daily diet). After 5 weeks of experiments, colorimetry was performed using Image Processing (10000*14000dpi quality). Image processing was carried out in Photoshop software using L*a*b system. The results showed that Spirulina treatment led to significant enhancement of L mean level at all treatments. Optimum ratio of 15% Spirulina in daily diet of Zebra Malawi cichlid was resulted in colour enhancement as all studied colour parameters was in significant difference in contrast to the control group(P
Ahmad Shadi; Hosein Zolgharnein; MohamadAli Salari-aliabadi; Mohamad Bagher Nabavi; Mohamad Taghi Ronagh
Abstract
Using 5 microsatellite loci Sillago sp. From Hormozgan North Persian Gulf were investigated through 68 specimens in two stations: Minab(31 specimens) and Bandar Lengeh(38 specimens). DNA was extracted using modified CTAB(Hexadecyl trimethyl-ammonium bromide) protocol. 5-17 alleles were observed in 5 ...
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Using 5 microsatellite loci Sillago sp. From Hormozgan North Persian Gulf were investigated through 68 specimens in two stations: Minab(31 specimens) and Bandar Lengeh(38 specimens). DNA was extracted using modified CTAB(Hexadecyl trimethyl-ammonium bromide) protocol. 5-17 alleles were observed in 5 loci with 8.4 mean allelic frequency. Observed and expected heterozygosity values was calculated (0.115<Ho<0.792) ,(0.598<He<0.902) . Genetic distance and identity according to Nei was estimated 0.520 and 0.595 respectively which shows genetic distance of genus level. Significant deviation from Hardy-Weinberg equilibrium (p<0.001) was observed at all loci at two stations. Analysis of Molecular Variance(AMOVA) of Genetic differentiation index(Fst) revealed mean significant (p=0.001) differentiation between two studied regions and 3.90 gen flow. Despite low distance of two studied regions (180km) there are probably two distinct populations of Sillago sp. which should be considered in management efforts.